Fig. 5.
Assessment of anti−tumour response in subcutaneous tumour models. C57BL/6 mice (n = 7) were subcutaneously injected with 2.5 × 105 OVA−B16F10−Luc cells. On the 7th and 14th days post tumour cells injection, tumour−inoculated mice were immunized via footpad injection with the indicated treatments, each contained 6 μg OVA. (A) Tumour growth curve and mice survival. (Left) Tumour growth monitored by calliper measurement. Values are expressed as mean value ± SEM. (Right) Tumour−inoculated mice survival. *P < 0.05, **P < 0.01, ***P < 0.001. (B) Histological analysis. The main organs and lymph nodes excised from scarified subcutaneous tumour inoculated mice stained with haematoxylin and eosin (H & E) (left) or neutral red (NR) (right). Images were captured at ×40 magnification. S−/+ appeared as dark black aggregates (arrows). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)