Fig. 2.

Na₂S and GYY4137 increased free sulfide while DATS increased bound sulfane sulfur. A: HUVECs were pre-incubated with SF7-AM for 30 min and rinsed with medium. Sulfide donors were given to cells at indicated concentrations. Fluorescent intensity was measured at different time points. * indicates significant difference between vehicle treatment at the same time point (n=6, p<0.05). B: HUVECs were treated with sulfide donors at indicated concentrations. Sulfide pools were measured by the MBB method. * indicates significant difference from vehicle treatment. # indicates significant difference from GYY4137 (50 μM and 500 μM) and DATS 5 μM. N=3–6, p<0.05.