Fig. 5.

CSE expression increased basal endothelial permeability. MAECs isolated from wildtype and CSE knockout mice and cultured on transwell inserts. A: Solute permeability was assessed by adding FITC-albumin to the top chamber and collecting medium at indicated time points over 4 h. * indicates significant difference between wildtype and CSE knockout MAECs treatment group at the same time point (n=3, p<0.05). B: Transendothelial resistance (TEER) across confluent MAECs on the inserts to determine ion permeability (n=3, p<0.05). CSE expression regulates junction and actin cytoskeleton structure. HUVEC were transfected with siRNA against CSE (siCSE). Mock treated and siCSE transfected cells were cultured to confluence. C: Immunofluorescence was performed for junction proteins and actin. D: Claudin 5 and CSE were analyzed by western blotting after transfection. * n=4, p<0.05.