Table 1.
Primers for quantitative real-time PCR.
| Gene | Forward | Reverse | Thermocycle | |
|---|---|---|---|---|
| F/C | Caspase-9 | 5′TCAGACATCGTATCCTCCA | 5′AGTCACAGCAGCACA | 98°C/3 min + 40 × [98°/15 s; 61°C/40 s] |
| P53 | 5′ACCTGCACTTACTCCCCGGT | 5′TCTTATAGACGGCCACGGCG | ||
| NF-κB | 5′AGGACTTAAAATGGCAGGAGAG | 5′GCTGTTCGTAGTGGTAAGTCTG | ||
| RIP1 | 5′GAACTAGGCTTCAGCAACTCCG | 5′GCAGCCAAAGAGGGCTTTGG | ||
| β-actin | 5′GGCACCCAGCACAATGAAG | 5′CCGATCCACACGGAGTACTTG | ||
|
| ||||
| 293T | Caspase-9 | 5′TGCTGAGCAGCGAGCTGTT | 5′AGCCTGCCCGCTGGAT | 98°C/3 min + 40 × [98°/15 s; 61°C/40 s] |
| P53 | 5′CCACCATCCACTACAACTACAT | 5′CAAACACGGACAGGACCC | ||
| NF-κB | 5′TCTCCCTGGTCACCAAGGAC | 5′TCATAGAAGCCATCCCGGC | ||
| RIP1 | 5′CATGGAAAAGGCGTGATACAC | 5′ACTTCCCTCAGCTCATTGTG | ||
| β-actin | 5′GGCACCCAGCACAATGAAG | 5′CCGATCCACACGGAGTACTTG | ||
F/C: fibroblasts/cardiomyocytes.