Figure 3.

Confocal Laser MicroScope (CLMS) to detect Vinculin and Integrin β1 in fibroblast periodontal ligament following treatment with medium enriched with sealants. Integrin β1: (a) untreated fibroblasts cultured for 12 h; (b) Biodentine- (BD-) treated fibroblasts cultured for 12 h; (c) Mineral Trioxide Aggregates- (MTA-) treated fibroblasts for 12 h, (d) untreated fibroblasts cultured for 24 h; (e) cultured fibroblasts treated with BD for 24 h; (f) fibroblast cultured with MTA-enriched culture medium for 24 h. Vinculin: (g) untreated fibroblasts cultured for 12 h; (h) BD-treated fibroblasts cultured for 12 h; (i) fibroblasts treated with MTA for 12 h; (j) untreated fibroblasts cultured for 24 h; (k) treated fibroblasts grown by BD for 24 h; (l) fibroblasts cultured with MTA-enriched medium for 24 h.