Figure 3.

Inhibiting PDE8 suppresses Teff cell adhesion to endothelial cells and is reversed by PDE4 inhibition. T cell blasts from C57BL/6 mice and bEnd.3 endothelial cells were incubated alone or in combination with IBMX (300 μM), PICL (1 or 0.1 μM), DP (100 μM) or PF-04957325 (1 or 0.1 μM). Values are normalized to the vehicle condition (0.1% DMSO) and presented as the mean ± SEM percentage of T cell blasts resistant to detachment. Data are averages from three to four independent experiments performed in triplicate (^*p < 0.05, ^**p < 0.001, one-way ANOVA and Bonferroni t-test).