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. 2016 Aug 23;6:31444. doi: 10.1038/srep31444

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A–C) CMPs were purified from Trib2^+/+ and Trib2^−/− mice as described in Fig. 3A and subjected to an in vitro colony-forming unit (CFU) assay for differentiation into myeloid and erythroid lineages. (A) Representative images of CFU plates are shown. The sorted Trib2^+/+ or Trib2^−/− CMPs were cultured in methylcellulose-based medium with recombinant cytokines and erythropoietin for 10 days. (B) The numbers of BFU-E derived from 200 sorted Trib2^+/+ and Trib2^−/− CMPs were recorded from plates in (A) (n = 6). (C) Bar graphs show the number of each different type of colony observed in plates (A). (D) 200 sorted MEPs from Trib2^+/+ and Trib2^−/− mice were cultured in vitro as described in (A), and the number of BFU-E was recorded (n = 4). The graphs show the mean and S.E.M. *P < 0.05 compares between the indicated groups. NS: no significant difference.