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. 2016 Aug 23;6:31506. doi: 10.1038/srep31506

Figure 5. Treatment with miR-214 inhibitor attenuated glomerular hypertrophy, accompanied by restoration of PTEN expression in db/db mice.

Figure 5

(A) Representative immunohistochemical staining for PTEN in glomeruli (original magnification ×400).Scale bars: 20 μm. (B) The mRNA levels of miR-214, PTEN, α-SMA and SM22 in isolated glomeruli detected by quantitative real-time PCR. (C) The protein levels of PTEN, α-SMA, SM22 and Collagen IV in isolated glomeruli detected Western blot. (D) Quantitative analysis of the protein levels of PTEN, α-SMA, SM22 and Collagen IV. db/m, db/m control mice; db/db, diabetic db/db mice; ScRNA, db/db mice treated with miR-214 scramble; anti-miR-214, db/db mice treated with miR-214 inhibitor. All data were expressed as means ± SD, ▲p < 0.05 vs. db/db group, ★p < 0.05 vs. ScRNA group.