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. 2016 Aug 23;6:31854. doi: 10.1038/srep31854

Figure 2. Total indolic glucosinolate content and relative expression levels of genes related to glucosinolate biosynthesis in double and triple myb mutant seedlings treated with glucose or sorbitol.

Figure 2

Effect of glucose on accumulation of total indolic glucosinolates (A) was measured in myb34myb122, myb51myb122, myb34myb51 double and myb34myb51myb122 triple mutants, as well as expression levels of MYB34 (B), MYB51(C), CYP79B2 (D), and CYP83B1 (E) in these mutants. Ten-day-old Arabidopsis seedlings were treated with 3% glucose or sorbitol, and then the whole plants were collected 3 days (A) or 18 h (B–E) after treatment. Each data point represents the mean of three to five (for qPCR assay) or six (for glucosinolate assay) independent biological replicates per treatment (mean ± SE). Expression level of genes in water-treated Col-0 seedlings was set to 1. Values not sharing a common letter are significantly different at P < 0.05.