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. 2016 Aug 23;6:31911. doi: 10.1038/srep31911

Figure 4. Immunofluorescence localization of BAMBI in peripheral CD4+ T cells.

Figure 4

Isolated CD4+ T cells were incubated with goat polyclonal Ab targeted against BAMBI and then were stained with TRITC-conjugated AffiniPure donkey anti-goat IgG (red). Colocalization of TGF-β receptors was visualized by (a) rabbit anti-TGF-β RI or (b) anti-TGF-β RII polyclonal antibody and FITC-labeled donkey anti-rabbit IgG (Green). DAPI staining solution was used for cell nuclei staining. The specificity of the immunostainings was demonstrated using isotype match controls (c).