Table 3. Primer sequences and product sizes of real-time PCR.
| Gene name | Primer sequence (5′–3′) | Product length (bp) |
|---|---|---|
| Foxp3 | F: CTGGCAAATGGTGTCTGCAAGT | 107 |
| R: CTGCCCTTCTCATCCAGAAGATG | ||
| RORC | F: CTGCAAGACTCATCGCCAAAG | 83 |
| R: TTTCCACATGCTGGCTACACA | ||
| TGF-β1 | F: GCGACTCGCCAGAGTGGTTA | 143 |
| R: GTTGATGTCCACTTGCAGTGTGTTA | ||
| TGF-β RI | F: GGACCCACTTCCATTTCCTTC | 125 |
| R: CCATCCCACTCCTCATCCA | ||
| TGF-β RII | F: GAAATTCCCAGCTTCTGGCTCA | 143 |
| R: CTGTCCAGATGCTCCAGCTCAC | ||
| BAMBI | F: CATACCCACATTGGAATGCTGTC | 144 |
| R: TGCACCTTGGTGATAAGGTTTCTG | ||
| Smad2 | F: GGCTTTACAGACCCATCAAATTCA | 161 |
| R: GCACTATCACTTAGGCACTCAGCA | ||
| Smad3 | F: CCAGGGCTTTGAGGCTGTCTA | 143 |
| R: GCAAAGGCCCATTCAGGTG | ||
| Smad4 | F: CAGCTATGCCAGAAGCCAGA | 81 |
| R:GAACTCCTGGGACTTTCAACTGAC | ||
| Smad6 | F: GGACAAAACAAGAAAGACGCACT | 83 |
| R: AAGGAAGAGGAAGGAGGAAGAGAA | ||
| Smad7 | F: CTGTCCAGATGCTGTGCCTTC | 126 |
| R: TATGCCACCACGCACCAGT | ||
| GAPDH | F: GCACCGTCAAGGCTGAGAAC | 138 |
| R: TGGTGAAGACGCCAGTGGA |
All of the primers were synthesized by TaKaRa Biotechnology (Dalian).