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. 2016 Aug 23;6:31668. doi: 10.1038/srep31668

Figure 4. Kremen1 modulates hair cell number in zebrafish neuromasts.

Figure 4

(A) A lateral view of a whole mount fish expressing Tg(cldnB:GFP). This fish has wild type distribution of neuromasts. Neuromasts O2 and P1 are labelled. (B) A lateral view of a whole mount fish mutant for Kremen1 and expressing Tg(cldnB:GFP). This fish has fewer neuromasts in the posterior tail region. Neuromasts O2 and P1 are labelled. Images provided by Drs H. McGraw and A. Nechiporuk. (C–E) Kremen1 mutants have excess hair cells per neuromast. (C) Mutants have a significant increase in hair cells in neuromasts O2 and P1. Asterisk (*) indicates p < 0.005. Hair cells (Parvalbumin, red) in neuromast O2 of a wild type fish (D) or a Kremen1 mutant fish (E). (F–H) Kremen1 over expression reduces hair cell numbers. (F) Fish injected with Kremen1 capped RNA have fewer hair cells per neuromast than control (phenol red) injected fish, asterisk (*) denotes p < 0.005. Hair cells (GFP) in neuromast P1, of a control injected (G) or Kremen1 capped RNA injected (H) fish. Scale bar indicates 10 μm. All error bars represent standard deviation.