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. 2016 Aug 23;6:31915. doi: 10.1038/srep31915

Figure 3. Long-term culture of glioblastoma TICs in 3D thermorevesible PNIPAAm-PEG hydrogel.

Figure 3

(a) The fold expansion and cell viability per 5 days at passage 1, 3, 5, 7, 9 and 10. (b) Phase images showing L0 spheroids in the hydrogel on day 1, 3, 5, 7 of the culture at passage 10. (c) Day 7 L0 spheroids released from the hydrogel at passage 10. (d) Live (green) and dead (red) staining of day 7 L0 spheroids at passage 10. (e) The fold expansion and cell viability of L0 and L1 TICs in the hydrogel on day 1, 3, 5, 7 and 9 of the culture at passage 10. (f–h) Immunostaining of day 5 L0 spheroids and (i) percentage of Nestin+, Ki67+, Tuj1+ and GFAP+ L0 and L1 TICs after 9 days culture in the hydrogel at passage 10. Images were taken after the spheroids were released from the hydrogels. Error bars represent the standard deviation (n = 3). ***Indicates statistical significance at a level of p < 0.001. Scale bar: (b,d) 250 μm, (c,f–h) 50 μm.