Figure 6. A scalable bioreactor for producing glioblastoma TICs.

TICs were mixed with PNIPAAm-PEG solution and processed into fibers. The fibers were suspended in a chromatography column. Cell culture medium was continuously perfused through the column. (a) A picture showing the column, pump and medium reservoir of the bioreactor. (b) A close look of the column with hydrogel fibers. Phase pictures showing one hydrogel fiber on day 3 (c) and day 7 (d) of the culture. The edges of the fibers were outlined with white lines. The individual spheroids were clearly seen in the fibers. Scale bar: 250 μm.