Skip to main content
. 2016 Aug 23;6:31931. doi: 10.1038/srep31931

Figure 1. Increased TIF in Sav-depleted kidneys after UUO.

Figure 1

(a) Western blot analysis of Sav1 protein expression in kidney lysates from WT and TEC-specific Sav1-null mice. Sav1 expression was abolished in TEC-specific Sav1-null mice after UUO. (*Indicates non-specific bands, and the arrow represents a verified Sav1 band). (b,c) TEC-specific Sav1 deletion enhances TIF after UUO. Masson’s trichrome staining in WT and TEC-specific Sav1-null mice showing increased extracellular matrix deposition within the tubulointerstitium at 7 days after UUO (b). Immunohistochemical staining for collagen IV in WT and TEC-specific Sav1-null mice showing increased expression of collagen IV at 7 days after UUO (c). (d,e) TEC-specific Sav1 deletions enhance TEC apoptosis and proliferation after UUO. Cell apoptosis and proliferation were examined by TUNEL assay (d) and PCNA immunostaining (e), respectively. TUNEL-positive cells and PCNA-positive cells were increased in injured TEC-specific Sav1-null mice (n = 5; *P < 0.01 versus kidneys of sham-operated WT mice; #P < 0.01 versus kidneys of sham-operated TEC-specific Sav1-KO mice; †P < 0.01 versus obstructed kidneys of WT mice at 7 days after UUO). Scale bar in b = 300 μm and c, d = 50 μm.