Skip to main content
. 2016 Aug 23;6:31686. doi: 10.1038/srep31686

Figure 1. Sub-cellular localisation, DNA binding activity and confirmation of transgenic tobacco plants.

Figure 1

(A) Gene construct RFP:SbSDR1, transient expression of RFP alone and RFP:SbSDR1 translational fusion protein in transformed onion epidermal cells; (B) DNA binding activity of SbSDR1 protein with genomic DNA of Salicornia brachiata and tobacco; (C) Schematic representation of SbSDR1-pCAMBIA2301 plant transformation vector construct; (D) Histochemical GUS assay; (E) Southern hybridization to determine transgene copy number, and (F) Semi-quantitative RT-PCR of transgenic lines along with control plants.