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. 2016 Aug 23;6:32007. doi: 10.1038/srep32007

Figure 3. Immunofluorescence staining of selected markers in SE and hiPSCs.

Figure 3

SE markers KRT8, KRT18, KRT19, p63, AP-2α, AP-2γ, ALDH1A3, p-SMAD1/5/9, CDH1, Desmoglein 3 and FOXG1 endodermal marker AFP, Mesodermal marker Brachyury (T), and neural ectoderm marker OTX2 were stained. DAPI was used to stain nuclei. The secondary antibodies Alexa −488 and Alexa −594 were used to visualize green and red signals, respectively. Insert in AFP staining image (+): Hela control cells stained by anti-AFP antibody and showed positive staining. Original magnification x200. Bars: 50 μm.