Table 1. Sequence of the oligonucleotide primer sets used in RT-PCR analysis.
| Target genes | Primer sequence 5′ to 3′ |
|
|---|---|---|
| Sense | Antisense | |
| Beta2microglobulin | CACTGACCGGCCTGTATGC | GGGTGGCGTGAGTATACTTGAATT |
| Cd36 | GGACATACTTAGATGTGGAACCCATA | TGTTGACCTGCAGTCGTTTTG |
| Fabp4 | TGTGGGAACCTGGAAGCTTGTC | TCTGACCGGATGGTGACCAAA |
| Fatp1 | GACAAGCTGGATCAGGCAAGC | AGTGAGGCCACAGAGGCTGTT |
| Fiaf | CAATGCCAAATTGCTCCAATT | TGGCCGTGGGCTCAGT |
| Glut1 | GGTGTGCAGCAGCCTGTGT | CACAGTGAAGGCCGTGTTGA |
| Glut4 | CCGGATTCCATCCCACAAG | CATGCCACCCACAGAGAAGA |
| G6pase | ACGTATGGATTCCGGTGTTTG | CAGCTGCACAGCCCAGAA |
| Inos | CACCTTGGACTTCACCCAGT | ACCACTCGTACTTGGGATGT |
| Il1beta | CAACCAACAAGTGATATTCTCGATG | GATCCACACTCTCCAGCTGCA |
| Npy | CAGAAAACGCCCCCAGAAC | CGGGAGAACAAGTTTCATTTCC |
| Pgc1alpha | AAAGGATGCGCTCTCGTTCA | GGAATATGGTGATCGGGAACA |
| Pomc | AGGCCTGACACGTGGAAGAT | AGCAGGAGGGCCAGCAA |
| Prdm16 | CAGCACGGTGAAGCCATTC | GCGTGCATCCGCTTGTG |
| Rpl19 | GAAGGTCAAAGGGAATGTGTTCA | CCTTGTCTGCCTTCAGCTTGT |
| Tnfalpha | TGGGACAGTGACCTGGACTGT | TTCGGAAAGCCCATTTGAGT |
CD36 (cluster of differenciation 36); Fabp4 (fatty acid-binding protein 4); Fatp1 (fatty acid transport protein 1); Fiaf (fasting-induced adipose factor); Glut1 (glucose transporter 1); Glut4 (glucose transporter 4); G6pase (glucose-6-phosphatase); Inos (inducible nitric oxide synthase); Il1beta (interleukin 1 beta); Npy (neuropeptide Y); Pgc1alpha (peroxisome proliferator-activated receptor 1 alpha); Pomc (pro-opiomelanocortin); Prdm16 (PR domain containing 16); Rpl19 (60S ribosomal protein L19); Tnfalpha (tumour necrosis factor alpha).