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. 2016 Aug 22;9(1):176. doi: 10.1186/s13068-016-0588-9

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Construction of vectors for the expression of A. cellulolyticus xylanases in C. bescii. a Schematic diagrams of Acel_0180 and Acel_0372 proteins: SP, signal peptide; GH10, a family 10 glycoside hydrolase; CBM3, a family 3 carbohydrate-binding module; CBM2, a family 2 carbohydrate-binding module. Maps of expression vectors for Acel_0180 (b) and Acel_0372 (c) expression. Genes were expressed under the transcriptional control of the C. bescii S-layer promoter. The expression vectors contain a CelA signal sequence, a C-terminal 6X his tag, a rho-independent terminator, the pyrF (from C. thermocellum) cassette for selection, and pBAS2 sequences for replication in C. bescii