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. 2016 Aug 22;9(1):176. doi: 10.1186/s13068-016-0588-9

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Confirmation of xylanase expression and activity in C. bescii. a SDS-PAGE analysis of concentrated extracellular proteins (20 µg). b Zymogram analysis of concentrated extracellular proteins (4 µg). M, Pre-stained SDS-PAGE standards, broad range (Bio-Rad Laboratories); 1, extracellular fraction from JWCB21 (parental strain); 2, JWCB73 (E1 expressing strain); 3, JWCB74 (E1 and Acel_0180 expressing strain); 4, JWCB75 (E1 and Acel_0372 expressing strain)