Table 1.
qPCR primers used to assess gene expression of markers of lipid/fatty acid metabolism
| Gene | Primer | Sequence 5′ _ 3′ | Accession numbera
(Probe number) |
|---|---|---|---|
| Chrebp | Forward Reverse |
AATCCCAGCCCCTACACC CTGGGAGGAGCCAATGTG |
NM_133552.1 (10) |
| D5d | Forward Reverse |
GAACTCTCTTCTGATTGGAGAGCTA CCGGAATTCATCAGTGAGC |
AB052085.1 (26) |
| D6d | Forward Reverse |
AATTTCCAGATTGAGCACCAC AGTGGGGCAATCTTGTGC |
NM_031344.2 (60) |
| D9d | Forward Reverse |
GAAGCGAGCAACCGACAG GGTGGTCGTGTAGGAACTGG |
NM_139192.2 (125) |
| Elovl2 | Forward Reverse |
AACCTCGGAATCACACTTCTTT TCCCAGCTGGAGAGAACG |
NM_001109118.1 (22) |
| Elovl5 | Forward Reverse |
TCGATGCGTCACTCAGTACC CCTTTGACTCGTGTGTCTCG |
NM_134382.1 (122) |
| Elovl6 | Forward Reverse |
ATGGATGCAGGAAAACTGGA GCCCGCTTGTTCATCAGA |
NM_134383.2 (119) |
| Fasn | Forward Reverse |
GGCCACCTCAGTCCTGTTAT AGGGTCCAGCTAGAGGGTACA |
NM_017332.1 (6) |
| Lxrα | Forward Reverse |
CAGGAAGAGATGTCCTTGTGG TCTTCCACAACTCCGTTGC |
NM_031627.2 (2) |
| Lxrβ | Forward Reverse |
AGCTCTGCCTACATCGTGGT GACCCTTCTTCCGCTTGC |
NM_031626.1 (106) |
| Srebf | Forward Reverse |
ACAAGATTGTGGAGCTCAAGG TGCGCAAGACAGCAGATTTA |
NM_001276707.1 (77) |
Forward and reverse primers for rat genes with GenBank Accession numbers and specific Roche Universal Probe numbers used for assessing mRNA expression in rat tissues by qPCR
aNational Center for Biotechnology Information (NCBI) EntrezGene (http://www.ncbi.nlm.nih.gov/gene)