Fig. 6.

S129 specific phosphorylation of human alpha-synuclein using PLK2 in vitro. HEK293 cells were transfected with dual expression plasmids coding for h-asyn or S129A h-asyn as well as PLK2 or the kinase dead mutant (mPLK2) (n = 6 per group; about 3 μg of protein loaded). Levels of total (open) and pS129 h-asyn (filled) of whole cell lysates were measured using the newly established AlphaLISA duplex assay and are presented in overlapping bars. Percentages of pS129 h-asyn to total h-asyn are indicated in the bottom of respective bars. **p <0.01; ##p <0.01; two-tailed Mann-Whitney test; Error bars indicate SEM