Fig. 8.

Assessment of S129 phosphorylated human alpha-synuclein in rat brain tissue. Quantification of pS129 h-asyn species after overexpression from rAAV6 vectors in the substantia nigra (a, b, d, e) or striatum (c, f). Tissue pieces (n = 6 per group) were processed sequentially with 1 % Triton (about 300 ng for striatal and 90 ng for VM samples of protein loaded) (a–c) followed by 1 % SDS (about 600 ng of protein loaded) (d–f) containing lysis buffer. Total (open) and pS129 (filled) h-asyn levels are presented in overlapping bars. Numbers inside the bars show the percent of pS129 to total h-asyn. **p <0.01; two-tailed Mann-Whitney test; Error bars indicate SEM