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. 2016 Aug 23;14(1):244. doi: 10.1186/s12967-016-1003-9

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — a Representative examples of stained muscle fibers in diaphragm and gastrocnemius muscles of control (top panel), U group (middle panel) and E–U group (bottom panel) in the 20-week cohort of animals. Myofibers positively stained with the anti-MyHC type II antibody appear in brown color (calibration bar 200 μm). Type I fibers appear in white color (not stained). b Representative examples of stained muscle fibers in diaphragm and gastrocnemius muscle of control (top panel) and U group (middle panel) and E–U group (bottom panel) in the 35-week cohort of animals. Myofibers positively stained with the anti-MyHC type II antibody appear in brown color (calibration bar 200 μm). Type I fibers appear in white color (not stained). anti-MyHC anti-myosin heavy chain, E–U elastase–urethane, U urethane