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. 2016 Aug 23;14(1):244. doi: 10.1186/s12967-016-1003-9

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — a Mean values and standard deviation of the percentage of abnormal fraction (white bars), cellular inflammation (grey bars) and internal nuclei (black bars) in the diaphragm muscle of the 20-week cohort animals. Statistical significance is represented as follows: *p ≤ 0.05 and ***p ≤ 0.001 between any of the intervention groups (U and E–U) and control mice. b Mean values and standard deviation of the percentage of abnormal fraction (white bars), cellular inflammation (grey bars) and internal nuclei (black bars) in the gastrocnemius muscle of 20-week cohort animals. Statistical significance is represented as follows: n.s.: non-significant and *p ≤ 0.05 between any of the intervention groups (U and E–U) and control mice. c Representative examples of muscle structural abnormalities in diaphragm of 20-week cohort animals (calibration bar 100 μm). A representative image of muscles in U group (right top image), in which black arrows point towards inflammatory cells, and a representative image of muscles in E–U group (right bottom image), in which black arrows point towards inflammatory cells and internal nuclei. d Representative examples of muscle structural abnormalities in gastrocnemius of 20-week cohort animals (calibration bar 100 μm). A representative image of muscles in U group (right top image), in which black arrows point towards inflammatory cells, and a representative image of E–U group (right bottom image), in which black arrows point towards internal nuclei. E–U elastase–urethane, U urethane