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. 2016 Aug 22;4(1):87. doi: 10.1186/s40478-016-0364-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Activation of microglia and astrocytes in the brainstem. a The histogram represents the number of Iba-1 positive microglia in the brainstem (n = 5–6 mice per group, ten pictures were counted per mouse). Note that aged-matched αSYN^tg/tg G3Terc^-/- mice (average 22.47 ± 2.32, n = 6) and αSYN^tg/tg mice (average 13.00 ± 0.67, n = 5) show a significant difference in number of microglia (p = 0.0058). b Representative pictures of Iba-1 staining in brainstem