Figure 3.

Cortical impact increases the density of neuroblasts in the gray matter and white matter dependent on lesion type. Piglets received cortical impact or sham surgery on PND 7 and the brain was collected at PND 14. The mean density of neuroblasts (average of eight fields per region per piglet) and max density of neuroblasts (field with the greatest density) in each region was compared in injured vs. sham piglets via Student's t-Tests. (A) Schematic illustrating the location of the cortical impact and resulting lesion in the rostral gyrus gray matter (blue) and rostral gyrus white matter (yellow). (B) The mean neuroblast density was not greater (P = 0.09) in injured vs. sham piglets. (C) The maximum neuroblast density was greater (^*P < 0.05) in the gray matter and tended to be greater (⁺P = 0.08) in the white matter of the rostral gyrus. (D) Piglets were binned into categories of lesion type based on pattern and extent, and neuroblast density was compared among groups via a one way ANOVA followed by Tukey-Kramer Multiple Comparisons Test. Injured piglets with a cavity⁺ lesion had a greater maximum density of neuroblasts than piglets with “cavity⁻” lesions (^{a, b}Means ± SEM with different letters differ, P < 0.05). (E) The mean number of BrdU⁺ cells per field was greater in the gray and white matter of injured vs. sham piglets (^*P < 0.05). (F) The mean number of BrdU⁺ neuroblasts per field was greater in injured rostral gyrus gray matter (^*P < 0.05), though the number is extremely low.