Table 2.
Results of the in-silico evaluation of the selected primer pairs.
| Primer pair | Pair name | TA(exp) | Reference | fragment length (nt) | Coverage of domain no MM | Coverage of domain 1 MM | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Archaea | Bacteria | Eukarya | Archaea | Bacteria | Eukarya | |||||
| S-D-Arch-0787-a-S-20/S-D-Arch-1043-a-A-16 | ArchV56 | 60 | Yu et al., 2005 | 272 | 76.50 | 0.00 | 0.00 | 84 | 0 | 0 |
| S-D-Arch-0519-a-S-15/S-D-Arch-1041-a-A-18 | ArchV46 | 60 | Ogawa et al., 2014 | 540 | 79.00 | 0.00 | 0.00 | 93.2 | 0 | 0 |
| S-D-Arch-0349-a-S-17/S-D-Arch-0786-a-A-20 | ArchV34 | 56 | This study | 457 | 74.00 | 0.00 | 0.00 | 90.7 | 0 | 0 |
| S-D-Arch-0519-a-S-15/S-D-Bact-0785-a-A-21 | PrkV4 | 60 | van Bleijswijk et al., 2015 | 287 | 88.60 | 88.20 | 0.70 | 95.2 | 95 | 1.4 |
| S-D-Bact-0007-a-S-20/S-D-Bact-0338-a-A-19 | BacV12 | 56 | Langfeldt et al., 2014 | 350 | 0.00 | 60.20 | 0.00 | 0 | 85.8 | 0 |
| S-D-Bact-0341-b-S-17/S-D Bact-0907-a-A-20 | BacV35 | 60 | Güllert et al., 2016 | 586 | 0.00 | 85.60 | 0.00 | 60.8 | 95.3 | 0.2 |
The TA temperature was applied in the amplification reaction. The Coverage of the primer pairs was estimated using the SILVA TestPrime tool with the sSU-NR database version 123 and refers to the percentage of potentially covered taxa in the different domains allowing no and one mismatch (MM) during primer annealing.