Table 1.

Primers used for the amplification and sequencing of the IHNV and VHSV full-length G gene.

Primer	Sequence 5′→3′	Position	Use	Amplicon size (bp)	References
VHSV
GB+	GTCGAAGAAGAGATAGGC	2796-2813a	RT-PCR	1171 bp	Einer-Jensen et al., 2004
GB-	GTTGGGTCGCCATGTTTCT	4548-4566a			Einer-Jensen et al., 2004
VHS Seq10 m-F	CCCTGGGCCTGGCAA	3975-3989a	Sequencing	–	This study
VHS Seq10 m-R	TTGCCAGGCCCAGGG	3975-3989a	Sequencing	–	This study
GSeq2+	GCCCATTGCCCCACG	3592-3606a	Sequencing	–	Einer-Jensen et al., 2004
VHS Seq2-R	CGTGGGGCAATGGGC	3592-3606a	Sequencing	–	This study
GSeq4+	CCTTGTGGAAGTCCCTC	4227-4243a	Sequencing	–	Einer-Jensen et al., 2004
VHS Seq4-R	GAGGGACTTCCACAAGG	4227-4243a	Sequencing	–	This study
GSeq6-	GCACAGAGTGACTTATCG	3258-3275a	Sequencing	–	Einer-Jensen et al., 2004
VHS Seq6-R	CGATAAGTCACTCTGTGC	3258-3275a	Sequencing	–	This study
IHNV
IHNVfl-FOR	CTCACTCCGTCCAAGACAG	2928–2946b	RT-PCR/Sequencing	782 bp	This study
IHNV-Rev1	CCTTCACGRCYCGATTGGAG	3690–3709b	RT-PCR/Sequencing
G1 FOR	AGAGATCCCTACACCAGAGAC	3523–3543b	RT-PCR/Sequencing	499 bp
IHNV-REV2	GATGTGGAGAKCGGAACTTG	4002–4021b	RT-PCR/Sequencing
IHNV IGSeq 5-F	GCACGCCGAGATAATATC	3954–3971b	RT-PCR/Sequencing	721 bp
IHNVfl-REV	GCCACCTTGTTCTTGTATC	4656–4674b	RT-PCR/Sequencing

Sequence, position of binding to the reference sequence, use and amplicon size are reported.

^aNucleotide positions where primers bind are in accordance with the sequence of VHSV strain 07–71 under the GenBank accession number AJ233396.

^bNucleotide positions where primers bind are in accordance with the IHNV sequence under the GenBank accession number X89213.