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Simulated CEST effect due to the presence of exchanging amide protons as a function of pulse duration and amplitude at 7 Tesla. The maximum amide proton transfer effect was found to occur with a pulse duration between 15 – 30 ms and pulse amplitudes of 0.5 μT – 3.0 μT. The saturation pulse parameters chosen for spinal cord CEST imaging were 25 ms duration and 2 μT amplitude (to account for B1+ effects).
