Fig. 3.—

SINE28 is RNA polymerase III dependent. (A) A multiple sequence alignment of all identified human SINE28s (table 1A) with putative RNA polymerase III promoter elements shown (supplementary table S2, Supplementary Material online). Below, DNA logos of predicted motifs with RNA polymerase III; conserved nucleotides are indicated by an asterisk. The degree of conservation among SINE28s is depicted at the bottom (black). (B) qRT-PCR of SINE28 (blue), the 18S (black), 5′-end of the 28S LSU (dark gray) and a portion in the middle of the 28S LSU (light gray) normalized to U1, pre and post RNA polymerase I inhibition (**P < 0.001). (C) qRT-PCR of SINE28 (blue) compared with a positive control target gene (gray) pre- and postspecific RNA polymerase inhibition (**P < 0.001). All data are normalized to an invariant transcript (control); confidence intervals (95%) are indicated. From left: RNA polymerase I inhibition—positive target 18SrRNA and control β-actin; RNA polymerase II inhibition—positive target β-actin and control 18SrRNA; RNA polymerase III inhibition—positive target U6 and control U1.