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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Nat Immunol. 2016 Jun 27;17(9):1037–1045. doi: 10.1038/ni.3509

Figure 3.

Figure 3

Sensing of bacteria transiently induces mitochondrial complex II and glycerol-3-phosphate dehydrogenase activities. (a, b, c, e, f) CII activity (a, b, c, e) and CII+CIII activity (f) in thioglycollate-elicited WT macrophages (a), CD14+CD16- human monocytes (b), or WT BMDMs (c, e, f) stimulated with EC or S. enterica Typhimurium (SL1344) (c) for 1.5h or for the indicated time. (d) Succinate (Succ)-driven ATP synthesis in WT BMDMs stimulated with EC for 1.5h. 100% activity corresponds to a rate of 70.4±18.1 nmol ATP/min/mg protein. (g-i) Mitochondrial glycerol-3-phosphate dehydrogenase (mG3PDH) (g, h) and G3PDH+CIII (i) in WT thioglycollate-elicited macrophages (g) or BMDMs (h, i) stimulated with EC for 1.5h or for the indicated time. NS, not significant; *P < 0.05, **P < 0.001 (two-tailed unpaired Student’s t-test). Data are means ± s.e.m. of three (a-c, h, i), four (f), six (e), or eight (d) independent experiments performed in three to five technical replicates, or one experiment with five mice per group (a) or three donors (b) and performed in two technical replicates.