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. Author manuscript; available in PMC: 2017 Jan 11.
Published in final edited form as: Nat Immunol. 2016 Jul 11;17(9):1075–1083. doi: 10.1038/ni.3495

Figure 2.

Figure 2

WNK1 is a positive regulator of T cell migration. (a) Migration of Jurkat T cells transfected with NT siRNA or an siRNA pool against WNK1 in response to CXCL12, normalized to CXCL12-induced migration of control cells. (b) Migration of mouse CD4+ T cells from top to bottom chamber of a Transwell plate in response to indicated concentrations of CCL21 in presence or absence of ICAM1. (c) Speed and displacement of mouse CD4+ T cells measured by time-lapse microscopy in response to indicated concentration of CCL21. Flower plots show overlay of migration tracks of individual T cells. *P < 0.01, **P < 0.0001 (Mann-Whitney). Sample sizes: a (5), b (6), c (567, control unstimulated; 67, control with CCL21; 450, mutant, unstimulated; 293, mutant with CCL21). Data are from one experiment representative of 2 (b,c) experiments or pooled from 5 (a) independent experiments. Graphs show mean±SEM.