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. Author manuscript; available in PMC: 2017 Jan 11.
Published in final edited form as: Nat Immunol. 2016 Jul 11;17(9):1075–1083. doi: 10.1038/ni.3495

Figure 7.

Figure 7

OXSR1, STK39 and SLC12A2 positively regulate chemokine-induced migration. (a) CXCL12-induced migration of Jurkat T cells transfected with the indicated siRNAs normalized to migration of cells transfected with NT siRNAs. Data for NT and WNK1 siRNA is the same as that shown in Fig. 2a and is repeated here for clarity. (b) CCL21-induced migration of mouse CD4+ T cells of the indicated genotypes. (c) CCL21-induced migration of mouse CD4+ T cells of the indicated genotypes. (d) Speed and displacement of mouse CD4+ T cells measured by time-lapse microscopy in response to CCL21. Flower plots show overlay of migration tracks of individual T cells. (e) CCL21-induced migration of mouse CD4+ T cells of the indicated genotypes in the presence or absence of bumetanide. *P < 0.01, **P < 0.001, ***P < 0.0001 (Mann-Whitney). Sample sizes: a (5), b (8), c (10, control cells; 11, mutant cells), d (630 control cells no CCL21; 1773 control cells with CCL21; 643 mutant cells no CCL21; 1105, mutant cells with CCL21), e (6). Data are representative of 2 (d) independent experiments or were pooled from 2 (b,e), 4 (c), or 5 (a) independent experiments. Graphs show mean±SEM.