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. 2016 Apr 1;241(14):1588–1602. doi: 10.1177/1535370216642528

Figure 8.

Figure 8

Immunofluorescent (IF) staining for identifying the expressions of Wnt1 and Wnt4 in kidney parenchyma at 72 h after IR procedure. (a) to (e) Illustrating the microscopic finding (400×) of IF staining for identification of Wnt1-positively stained cells (green color) mainly in glomeruli. (f) Analytic results of Wnt1+ cell expression, SC vs. IR, p < 0.0001; * vs. other groups with different symbols (†, ‡, §, ¶), p < 0.0001. (g) to (k) Illustrating the microscopic finding (400×) of IF stain for identification of Wnt4-positively stained cells (green color) mainly in renal tubules. (l) Analytical results of Wnt4+ cell expression, SC vs. IR, p < 0.0001; * vs. other groups with different symbols (†, ‡, §, ¶), p < 0.0001. Scale bars in right lower corner represent 20 µm. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n=8 for each group). Symbols (*, †, ‡, §, ¶) indicate significance (at 0.05 level). Ex4: exendin-4; IR: ischemia–reperfusion; Mel: melatonin; SC: sham control. (A color version of this figure is available in the online journal.)