Effects of ODN2006 stimulation on the mRNA and protein expression levels of PMEL, TYR, and TLR9 in PIG1 melanocytes. After serum starvation for 24 h, PIG1 melanocytes were treated with 5 µM ODN2006 for 0, 6, 24, and 72 h, and then subjected to respective detection. (a) The mRNA levels of PMEL, TYR, and TLR9 were evaluated using real-time RT-PCR. (b) The protein expression levels of PMEL, TYR, and TLR9 were evaluated using Western blotting