Figure 1.

Experimental design. (a) The general scheme of successive experiments performed. In each experiment, mice were treated for seven days with each respective antidepressant drug. To assess humoral immune response, thioglycollate-induced peritoneal macrophages (TMfs) collected from these mice were pulsed with SRBCs and transferred intraperitoneally into drug-untreated recipients. For evaluation of active contact sensitivity (CS) reaction, drug-treated mice were contact sensitized, then challenged with PCL and elicited CS was measured as ear swelling response. Oil-induced peritoneal macrophages (OIL Mfs) from drug-treated mice were tested either for expression of surface markers in cytometry, generation of ROIs in a chemiluminescence assay, or for secretion of cytokines and nitric oxide during cell culture. Finally, drug-treated mice were the source of OIL Mfs, which, after conjugation with trinitrophenyl (TNP) hapten (TNP-Mfs) were transferred intravenously into mice that were then epicutaneously (e.c.) sensitized and subsequently challenged with PCL to estimate CS reaction measured as ear swelling response in TNP-Mf recipients. (b) Scheme of experiment testing for early 2-h and late 24-h phases of active CS reaction in antidepressant drug-treated, e.c. sensitized mice. (c) Presentation of the model of intravenous adoptive transfer of TNP-Mfs from antidepressant drug-treated donors into naive recipients one day before PCL e.c. sensitization for testing of drug-influenced TNP-Mf impact on early and late phases of CS reaction measured as ear swelling response after challenge with PCL. (A color version of this figure is available in the online journal.)