Figure 3.

Effects of ITD on the translocation of Nrf2 and the inaction of Keap1. (a) HepG2 cells were incubated with ITD (32 µM) for 3 h, 6 h and 18 h. (b) HepG2 cells were treated with various concentrations of ITD (8, 16 and 32 µM) for 6 h, the levels of proteins were examined by Western blot analysis. (c) Cells were incubated with ITD (32 µM) for 1 h, 3 h and 6 h, and Western blot analysis were employed to analyze the nuclear and cytoplasmic levels of Nrf2. Each bar was expressed as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01 versus control group.

ITD: isotetrandrine; Keap1: Kelch-like ECH-associated protein 1