Figure 2.

MiR-152 expression in C33A cells, and tumor and non-tumor cervical tissues, and the effect of HIF-1α siRNA. (A) (a) The expression of miR-152 in C33A cells, and the effect of HIF-1α siRNA on this expression, was determined by qRT-PCR. Data are presented as means ± SD (**P < 0.01) (n = 3). (b) The expression of miR-152 tumor and non-tumor cervical tissues was determined by qRT-PCR (*P < 0.05). (B) miR-152 expression was assayed by qRT-PCR using RNAs harvested from HeLa cells treated with siRNAs against HIF-1α (siHIF-1α), HIF-2α (siHIF-2α), or a scrambled control siRNA (siSRC) under normoxia (N) or 2% O₂ (H) for 24 h. RNA levels were normalized against small nuclear RNA U6. Representative blots of three replicates are shown. Data are presented as means ± SD