Table 1. Linker-modified variants of CcsA and Syn2^a.

Strain	Linker modificationb	Linker length (amino acid)
CcsA WT	None	150
CcsA-CAC	Linker swap–A. nidulans AN8412	105
CcsA-CEC	Linker swap–A. clavatus ACLA_023380	69
CcsA-CMC	Linker swap–M. oryzae CAG28798	163
CcsA-LΔ150	Deletion in the central part of the linker	100
CcsA-LΔ225up	Deletion of the N-terminal end of the linker	75
CcsALΔ225dw	Deletion of the C-terminal end of the linker	75
CcsA-L-GSG	Substitution of the linker for Gly-Ser-Gly	3
Syn2-L-GSG	Substitution of the linker for Gly-Ser-Gly	3
CcsA-RFPlink1	Insertion of RFP in the linker	373
CcsA-RFPlink2	Substitution of the linker for RFP	235

^a See also S3 Fig.

^b PKS- and NRPS domains were predicted using the NCBI Conserved Domain Database [43] and the linker was defined between the ACP domain and the condensation domain at positions 2487–2636 (amino acid sequence).