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. 2016 Aug 23;11(8):e0161697. doi: 10.1371/journal.pone.0161697

Fig 4. Determination of the thermal stability of the TYR variants by thermal shift assays.

Fig 4

Fluorescence scan diagrams based on Sypro Orange (Thermo Fisher Scientific) binding upon thermal unfolding of TYR456 and TYR469 proteins, respectively. The apparent stability midpoint values (or melting temperatures) under the analysed buffer conditions are 72°C and 60°C for TYR456 and TYR469, respectively.