Fig 7. Transplant of Abca12^smsk/smsk skin grafts.

(a) Back skins of E18.5 WT and Abca12^smsk/smsk embryos were transplanted onto nude mice. Bar = 2 mm. (b) Histological presentation of H&E stained sections of the grafts from WT and smsk transplanted skin. Note the loss of normal epidermal architecture at the end of 3 wks of transplantation in the smsk grafts. Bar = 50 μm. (c) Smsk E18.5 skin grafts were treated daily with a cream containing either the recombinant KLKs or the digestion buffer (Control). Shedding of the top layers of the hyperkeratotic skin grafts was observed only in grafts treated topically with KLK cream. Images were taken after 7 days of treatment. Bar = 2 mm. (d) Representative H&E images are shown of control and KLK cream treated smsk mutant skin grafts. Bar = 10 μm.