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. 2016 Aug 23;11(8):e0161463. doi: 10.1371/journal.pone.0161463

Fig 2. Sciatic nerve MHC-I expression by distinct cellular sources after crushing.

Fig 2

Double immunolabeling of MHC-I molecules and A) neurofilament (NF, axonal marker), or B) S100 protein (Schwann cell marker) or C) Iba1 protein (macrophage marker) after crushing. A) Most of the axons expressed zero or low MHC-I immunolabeling. Inset: MHC-I+ axon (arrowheads). B) MHC-I expression by Schwann cells. Inset: MHC-I labeled Schwann cell (arrowheads). C) Macrophages expressing MHC-I after injury. In detail, MHC-I labeled macrophages (arrowheads), one of which with cytoplasmic MHC-I immunolabeling. D) MHC-I expression by endothelial cells. A blood vessel is highlighted (dashed lines). Nuclei are DAPI positive, NF: neurofilament, CD31: endothelial cell adhesion molecule. Time-points: A and D, 4 weeks after lesion (wal); B and C, 2 wal. Scale bars: A and D, 50 μm; B and C, 100 μm; insets, 20 μm.