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(A) to (D) Flow cytometric analysis showed that CPX (10 μM) clearly induced G1 cell cycle arrest in all the 4 cell lines of T-ALL, especially after 12 h treatment. (E) Western blot analysis indicated that CPX (10 μM) markedly induced upregulation of CDK inhibitor of p21, but inhibited CDK inhibitor of p27, cyclin D, retinoblastoma protein (Rb), and phosphorylated Rb (pRb) in CEM-C1 cells.
