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. Author manuscript; available in PMC: 2016 Aug 23.
Published in final edited form as: Crit Care Med. 2011 Jul;39(7):1749–1759. doi: 10.1097/CCM.0b013e3182190b62

Figure 7.

Figure 7

Calpain and caspase-3 activity in the diaphragm from control (CON) and mechanically ventilated animals with (MVSS) without mitochondrial-targeted antioxidants (MV). A) Levels of the 145 kDa α-II-spectrin break-down product (SBPD) in diaphragm muscle following 12 hours of MV. Note that the SBDP 145 kDa is an α-II-spectrin break-down product that is specific to calpain cleavage of intact α-II-spectrin and therefore, the cellular level of SBDP 145 kDa can be used as a biomarker of in vivo calpain activity. B) Levels of the 120 kDa α-II-spectrin break-down product (SBPD 120 kDa) in diaphragm muscle following 12 hours of MV. Note that the SBDP 120 kDa is a α-II-spectrin break-down product that is specific to caspase-3 cleavage of intact α-II-spectrin and therefore, the cellular levels of SBDP 120 kDa can be used as a biomarker of caspase-3 activity. The images above the histograms in Figure 7A and 7B are representative western blots of data from the three experimental groups. Values are means ± SEM. * = different (p<0.05) from both CON and MVSS. ‡= different (p<0.05) from MVSS (n=6/group).