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. 2016 Aug 22;213(9):1851–1864. doi: 10.1084/jem.20150514

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© 2016 Sun et al.

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Figure 6. — Influenza and MRSA coinfection induces both inflammatory monocytes and neutrophils into airways. (A–C) Mice were infected with PR8 and 7 d later superchallenged with MRSA. BALF cells from WT and Nox2-deficient (KO) mice were analyzed for numbers of CD11b⁺ myeloid cells or neutrophils at days 1 (A), 3 (B), and 6 (C) post-infection. *, P < 0.05; ***, P < 0.001, Student’s t test. (D) Flow cytometry analysis of airway inflammatory monocytes (Mo) and neutrophils (PMN) in coinfected CX₃CR1^EGFP mice (n = 4) at 6 dpi. (E) BALF cells from WT and Nox2-deficient (KO) mice were analyzed for the compositions of CD11b⁺ myeloid cells (mean of five mice/group) at 6 dpi. All infected mice were treated daily with linezolid until 24 h before harvesting the samples. Data shown are representative of at least two independent experiments.