Figure 7.

Nox2 activity during coinfection reduces inflammatory cell survival. (A–G) WT and Nox2-deficient (KO) mice were infected with PR8 and 7 d later superchallenged with MRSA. (A–D) CD11b⁺ BALF cells (n = 4–5) were analyzed by flow cytometry after staining with annexin V and FVD at the time of isolation (0 h), as well as 24 h after in vitro culture. *, P < 0.05; **, P < 0.01, Student’s t test. (A) Numbers indicate the mean percentages of four mice/group. (E) Diff-Quick–stained BALF cells from day 3 coinfected WT and Nox2-deficient (KO) mice and after 24 h in culture. Bars, 50 µm. (F and G) At 3 dpi, CD11b⁺ BALF cells were stained with annexin V and FVD after 24 h in culture with IM-54, necrostatin 1 (Nec-1), or solvent control (DMSO). (F) BALF cells pooled from at least three mice per group are shown (mean ± SD of triplicate wells). *, P < 0.05; **, P < 0.01, paired Student’s t test. All coinfected mice were treated daily with linezolid (A–D) or gentamicin (E–G) until 24 h before harvesting the samples. Data shown in (A–F) are representative of at least two independent experiments. Data shown in G were combined from four independent experiments. Error bars represent SD.