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. 2016 Aug 22;213(9):1779–1798. doi: 10.1084/jem.20160340

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© 2016 Gay et al.

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Figure 7. — IFN-γ–induced activated STAT1 DNA association is enhanced by T. gondii in a TgIST-dependent manner. (A–C) HFFs were infected for 24 h with Pruku80 (WT) and Pruku80 ΔTgIST (ΔTgIST) or left uninfected (ui) and stimulated 6 h with IFN-γ (+) or left unstimulated. Samples were analyzed by ChIP assay with antibodies to STAT1 Y701-P, STAT1 S727-P, and H3K4me3. IgG was used as negative control. Bound DNA corresponding to IRF1, CXCL10, and CIITA loci was quantified by qPCR-ChIP, and signals were normalized with the input DNA. Error bars represent SD (n = 3). Data are from one representative of three independent experiments. (D) ChIP-qPCR was used as aforementioned to monitor enrichment of H3K9ac, H4panAc, and H3K27ac at IRF1, CXCL10, and CIITA promoters. Error bars represent SD (n = 3). Data are from one representative of two independent experiments.