Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 24;7:1329. doi: 10.3389/fmicb.2016.01329

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Fang, Zhao, Liu, Wang, Duan and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Detection of the suppression of the transgene-induced silencing and dsRNA-binding activity of 2b and its mutations. (A) Purified GST-tagged 2b, 2b_(8–111), and 2b_(18–111). (B) Gel mobility shift assays for the detection of the dsRNA binding affinity. GST-tagged 2b, 2b_(8–111), and 2b_(18–111) were incubated with siRNA duplexes and long dsRNA. 2b_(8–111) showed a high affinity for 21/24-nt siRNA duplexes, similar to 2b, but showed a reduced affinity for long dsRNA; 2b_(18–111) showed no binding to 21/24-nt siRNA duplexes. (C) GFP fluorescence in the leaves of Nb plants coinfiltrated with GFP and 2b or mutants. Coinfiltration of GFP with vector was used as control. Photographs were taken under UV light at 4 dpi.