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. 2016 Aug 24;7:1329. doi: 10.3389/fmicb.2016.01329

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Copyright © 2016 Fang, Zhao, Liu, Wang, Duan and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Colocalization pattern of 2b or its derivative mutants with AGO4 in Nb leaf epidermal cells. (A) Subcellular localization of 2b-EGFP, 2b_(8–111)-EGFP, 2b_(18–111)-EGFP, and RFP-AGO4 in Nb leaf epidermal cells. DAPI staining was performed to represent the nuclei. Bars = 7.5 μm. (B) Subcellular location of the coexpression of 2b-EGFP, 2b_(8–111)-EGFP, or 2b_(18–111)-EGFP with RFP-AGO4. (C) Diagram of the 2b function domain as previously reported (Duan et al., 2012). NLS, nuclear localization signal.